
Edina Blazevic
Research Scientist
Summary
PROFESSIONAL QUALIFICATIONS
Collaborator: Motivated, curious, and enthusiastic with a passion to collaborate with cross functional teams to drive improvement and lead change within a multidisciplinary organization
Research and Experimental Design: Experienced in design, product development and validation of life sciences instrumentation, with excellent organizational skills | Knowledge in bio-threat agent detection and hands-on biology-based assays and techniques | laboratory experiences performed both on the lab bench and with automated instruments
Mentor: Academic Instructional and laboratory knowledge in Hematology and Histology with experience with cell culture, cell sorting, manipulating of primary cells, processing diverse sample types (tissue, blood, biopsies) immunohistochemistry staining
Techniques: Cell isolation, cultivation, identification, culturing, counting, and staining, aseptic techniques, Simple/Acid Fast/Gram/ Endospore and Flagella stains, Confocal microscopy, bright-field, fluorescence and spectral imaging microscopy, PCR primer design, DNA/RNA isolations, Extraction of plasmid DNA, ELISA, Flow Cytometry/Gating, Agarose Gel Electrophoresis, Northern/Southern/Western hybridization; Blood smears, complete blood and platelet count (manual and automated), blood typing, IHC staining. tissue sectioning and processing
Instruments: Incubator, Autoclave, Centrifuge, Analytical Balance, Bioanalyzer, Microplate Reader, Slide Stainer, Microtome, Cryostat, Spectrophotometer, UV Cross Linker, Real Time Thermal Cycler, Fluorometer, Anaerobic Gas Chamber, Bright-field, Fluorescence and Spectral Confocal Microscope, Multispectral Imager and BSL-1 and BSL-2 tissue culture suites
Software: Microsoft Excel, Jmp (Statistical analysis software), MS office, DocuSign, Adobe Acrobat, Oracle Procurement Cloud (Vendor management), NetSuite (Inventory management), Gemident, Nuance (Multispectral imaging and scanning software), Arena Solutions (Web-based Product Lifecycle Management (PLM) Document Control), Wrike (Product management and collaboration software), Deltek (Enterprise Resource Management (ERP) software system for government contractors), Aestiva (Web based purchase order and accounting system) and GenePix® Pro 7 Software (Microarray Image Analysis Software)
Experience
SENIOR SYSTEMS INTEGRATION ENGINEER
Roche Sequencing Solutions / Santa Clara, CA
- Developed, maintained, and ran the baseline protocols for the Nanopore Sequencers in order to track and analyze the system performance on a weekly basis.
- Integrated new research advances into the baseline by analyzing raw data, conducting statistical analysis using data software tools (JMP), and presenting analysis reports.
- Collaborated with diverse functional teams and subject matter experts on interdisciplinary projects to optimize sequencing performance.
- Applied advanced technical writing skills to construct SOPs and documentation.
- Processed and prepared necessary equipment and reagents for sequencing experiments using mechanical and chemical/wet lab techniques.
Research and Development Associate IV
General Automation Lab Technologies, Inc / San Carlos, CA
- Developed workflows to support microbial identification in GALT’s array-based technology including the translation, development and validation for a variety of developmental test using GALT’s platform.
- Supported the instrument development of the array-based technology using the Prospector platform and conducted experiments and POP (proof of principle) to ensure prototype viability by verification testing
- Developed and assessed SOPs and maintained document production by completing forms, logs, reports and records of both internal and customer performed tests
- Selected 30 + medically relevant fungi and bacterial strains for the instrument development projects
- Cultured, maintained, and determined growth parameters of microbial strains
- Optimized and tested numerous bacterial nutrient medias to culture unculturable microorganisms
- Managed and maintained BSL-2 laboratory including reagent and consumable inventories for product development testing and production
- Worked with outside vendors/partners to accelerate product development including the standard bacterial and fungal isolation/purification/preservation from environmental samples, culture media preparation, serial dilutions, laboratory stock solution/reagent preparation, aseptic technique and Sanger sequencing library preparation.
Experiment Support Scientist
NASA AMES Research Center / Moffett Field, CA
- Involved in the space flight experiment on the discovery flight at the Kennedy Space Center, Florida.
- Conducted experiments that looked for expression level variations in proteins
- Worked on RNA Sequencing, Q-RT-PCR, RNA Isolation, and Northern analysis procedures using equipment such as: Centrifuge, Spectrophotometer, UV cross linker, Autoclave and Q-RT-PCR machines.
- Maintained laboratory stocks and other experimental laboratory solutions.
Scientist
PositiveID Corporation / Pleasanton, CA
- Conducted test validations, performance optimization and maintenance of a flow-through sample prep unit in a biological pathogen sensor using several bio-defense pathogens
- Appointed as the point of contact person liaising for all collaborative research and development assignments
- Managed the procurement process for biological purchasing, receiving and inventory management for the accounts payable process
- Coached and mentored staff to ensure each project or program was meeting proposal conditions and expectations
- This highly demanding role requires extremely detail oriented, well organized, bright and independent self-starters with solid knowledge of specific biological samples in the air and clinical samples,
- Conduct the PCR cloning successfully on several bio-defense pathogens including Anthrax, human papilloma virus, H1N1 and other forms of the influenza virus.
- Involved in the HTAC testing and performance
Life Science Research Associate III (LSRA) / Histologist / Laboratory Manager
Stanford University, Department of Medicine Division of Hematology / Palo Alto, CA
- Acquired tumor, tumor-draining lymph nodes (TDLN) and blood samples for analyses and furthered optimized methods for analysis of fresh and archived samples by using the IHC techniques.
- Developed multicolor tissue IHC staining protocols, executed, and analyzed over 100 samples using 3 and 4 color immunohistochemical (IHC) stain panels for maximum recover of all cells from tumor and TDLN specimens.
- Assisted in optimizing the development of custom image analysis software (Gemident) to identify each cell, cell type, location, and relate cell populations by distances and geometric patterns.
- Supervised, mentored, and trained 3 undergraduate and 2 graduate students
Hematology Laboratory Lecturer
San Jose State University, Department of Biology / San Jose, CA
- Provided lecture and hands-on training for laboratory exercises relative to in-depth study of blood and blood forming organs including normal and pathological blood forms, developed quizzes and graded assignments for graduate students
- Taught sterile and safety techniques, blood smear preparation, acid-fast staining, microscopy use and cell identification and manual enumeration methods
- Developed strategies, processes, and recommendations by applying principles, methods, and knowledge to develop and meet the overall strategic goals
- Increased overall awareness and education by implementing new programs through workshops, skill enhancement program and team building activities
Education
San Jose State University
Bachelor of Science, Molecular Biology
Bachelor of Science, Molecular Biology
(2003) San Jose State University